 Contact 3315 MSRB III SPC5606, 1150 W. Medical Center Drive Ann Arbor, MI 48109-0600 mbwolfe@umich.edu Github: https://github.com/mikewolfe Education M.S. in Bioinformatics from the University of Michigan, 2015-Present B.S. in Biomedical Engineering from The Ohio State University, 2014 Research Interests I work on understanding gene regulation at a global level, mainly in bacteria but also in higher eukaryotes (humans), by probing the interactions of Nucleic Acid binding proteins and their RNA or DNA targets. My approaches to this mainly include the analysis of large-scale high-throughput sequencing datasets to create regulatory maps of particular transcription factors (proteins that bind to DNA and control transcription and thus RNA expression), or RNA binding proteins (proteins that bind to RNA and control RNA stability/translation and thus RNA and protein expression). In addition to the analysis of experimental data, I am also developing computational algorithms to predict where Transcription Factors or RNA binding proteins bind given nucleic-acid sequence information only. The overarching end goal of this effort is to reverse engineer the regulatory systems of cells so we can predict what they will do in an environment of interest, target maladaptive regulatory states and change them (antibiotics for bacteria, disease states for humans), or create regulatory systems in bacteria to make products of interest. Publications *Indicates co-first authorship Wolfe M. B., Goldstrohm A. C., Freddolino P. L. Global Analysis of RNA metabolism using bio-orthogonal labeling coupled with next-generation RNA sequencing. Methods (2018). in press *Kroner, G. M., *Wolfe, M. B. and Freddolino, P. L. Escherichia coli Lrp regulates one- third of the genome via direct, cooperative, and indirect routes. Journal of Bacteriology JB.00411-18 (2018). doi:10.1128/JB.00411-18 in press Fellowships and Awards National Science Foundation
University of Michigan
Professional History 2015 to Present: Graduate Research Assistant, Freddolino Lab, Biological Chemistry Department, University of Michigan 2010 to 2014 Undergraduate Research Assistant, Jackman Lab, Department of Chemistry and Biochemistry, Ohio State University |
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